What is NAD+?

NAD⁺ (Nicotinamide Adenine Dinucleotide) is a coenzyme that occurs naturally in every living cell. It helps regulate energy production and cellular balance. Over time, natural NAD⁺ levels decline, which is why NAD⁺ supplementation — including injections, capsules, and IV therapy have become a popular area of interest in the longevity and wellness space.

What are the benefits of NAD+ injections?
  • At-home convenience: precise, repeatable dosing without clinic visits.
  • Direct delivery: subcutaneous administration for efficient uptake (no digestive step).
  • Quality assurance: EU-GMP manufacture, full third-party testing, published COAs.
  • Simple protocol: minimal, clinical-grade kit with clear instructions.

Where should I inject NAD+?

Common subcutaneous injection sites for NAD⁺ include:

  • Abdomen (stomach): the soft area around the belly, at least 2–3 cm away from the navel.
  • Outer thigh: the fleshy outer portion of the upper leg.
  • Upper buttock or hip: the upper outer quadrant where there is a thin layer of soft tissue.

If you’re unsure, follow the step-by-step guide in your kit or ask the practitioner who supplied your protocol for site selection and technique.

How do I inject using the vial and syringe?

NAD⁺ is given as a subcutaneous injection — just under the skin.

  1. Wash your hands and prepare a clean surface.
  2. Wipe the vial top and chosen injection site with an antibacterial swab.
  3. Draw up the correct dose using a fresh syringe.
  4. Pinch a small fold of skin (stomach, thigh, or buttock).
  5. Insert the needle at the angle shown in your kit guide and inject slowly.
  6. Dispose of the used syringe immediately using the needle safe.

Always follow the instructions supplied in your kit. If you are unsure about self-injection, seek guidance from a trained professional before starting.

For a video guide please see 'How To Inject'.

How do I inject using the Bio Pen auto-injector?

For a video guide please see 'How to Inject'

Are NAD+ injections safe?

NAD⁺ is a molecule that naturally exists in every cell of the body. Our injections are manufactured in Europe to EU-GMP standards, independently tested, and supplied with Certificates of Analysis (COAs) to verify purity and quality.

When used as directed, most people only experience mild, short-lived effects such as slight redness or stinging at the injection site.

NAD⁺ injections are not recommended for:

  • Individuals under 18
  • Those who are pregnant or breastfeeding
  • Anyone with certain medical conditions (see our contraindications FAQ for details)

If you have a health condition or take prescription medication, consult a healthcare professional before beginning NAD⁺ supplementation.

Who should not use NAD⁺ injections?

You should avoid NAD⁺ injections, or first seek medical advice, if you have:

  • A history of cancer, are undergoing cancer treatment.
  • Lyme disease
  • A recent skin graft
  • Atrial fibrillation
  • Immunosuppression
  • A history of organ transplant
  • Haemophilus influenza or other NAD-dependent bacterial infections
  • Pregnancy or are breastfeeding
  • Under 18 years of age

If you are managing any other health condition or taking prescription medication, consult a qualified healthcare professional before starting NAD⁺ injections.

How often should I take NAD+ injections?

The frequency depends on your personal goals and wellness plan. Some people begin with daily injections for an initial phase, then reduce to weekly or as-needed for maintenance. Always follow the protocol provided with your kit or seek guidance from a qualified practitioner.

How long does it take to feel the effects?

Everyone responds differently. Some people notice changes in focus or energy within days, while others experience more gradual effects over weeks. Even if you don’t feel an immediate difference, NAD⁺ is still absorbed and used at the cellular level.

What is the Needle Safe?

The NeedleSafe is a compact device designed for safe and easy syringe disposal. It works with the Nevershare and BBraun Omnican syringes.

  • With a single click, the used needle locks into one of 20 secure chambers.
  • The top rotates to hide the used needle and reveal the next chamber.
  • Once all 20 are used, the NeedleSafe can be returned for disposal or placed in domestic waste (as the contained needles pose no risk).

👉 Watch how it works here

Is NAD+ the same as Vitamin B3?

Not exactly. Vitamin B3 (niacin or niacinamide) is a precursor that the body can convert into NAD⁺. However, this process is not always efficient, and levels decline with age. Direct NAD⁺ supplementation provides the molecule itself rather than relying on conversion from B3.

Why supplement with NAD+ instead of B3?

While Vitamin B3 supports the body’s production of NAD⁺, taking NAD⁺ directly provides the molecule in its active form. This bypasses the conversion process, which can be less effective as we get older.

Can NAD+ help with anti-aging?

Research shows that NAD⁺ is involved in many cellular processes linked to energy, repair, and balance. While studies continue, NAD⁺ is widely explored in the field of longevity science. Many people choose NAD⁺ as part of a broader wellness routine aimed at supporting healthy aging.

Can I combine NAD+ injections with other supplements or treatments?

Yes, NAD⁺ is often used alongside other wellness supplements such as L-glutathione, methylene blue, or CoQ10. However, if you are on prescription medication or undergoing medical treatment, speak to your doctor before combining protocols.

How does NAD⁺ compare to NMN or NR supplements?

NMN (Nicotinamide Mononucleotide) and NR (Nicotinamide Riboside) are precursors — the body converts them into NAD⁺ through several steps. This process can be less efficient, especially as we age.

Direct NAD⁺ supplementation delivers the molecule itself, avoiding conversion and potential variability. At Bio Atelier, we focus on pharmaceutical-grade NAD⁺ with full third-party testing to ensure purity, stability, and consistency.

How do I store NAD+ injections?

Keep the vial refrigerated and stored upright. Avoid freezing and protect it from direct light or heat.

Do NAD+ injections hurt?

What Does It Feel Like?

  • Most people describe it as a quick pinch or slight pressure.
  • Any discomfort usually lasts just a few seconds.
  • Some may feel a mild stinging sensation if injecting too fast.

Tips to Make It More Comfortable:

  • Numb the area – Use a cold compress or numbing cream before injecting.
  • Inject slowly – A slower injection can reduce any stinging sensation.
  • Choose the right site – Fatty areas like the abdomen or thigh tend to be less sensitive.

If you’re still unsure, consider having a professional administer your first injection to help ease your anxiety. OR you could try our liposomal capsules.

Why do high-purity NAD⁺ products cost more


Industry experts warn: “If a supplement seems too good to be true, it usually is.” The difference comes down to standards. Our pricing reflects pharmaceutical-grade synthesis, zero fillers, EU-GMP manufacturing, and full third-party testing — safeguards that cheaper products often skip.

Can I accurately measure my NAD⁺ levels?
  • Direct testing is still evolving. Current methods are fragile because NAD⁺ degrades quickly once sampled.
  • Levels also fluctuate naturally during the day (often peaking mid-afternoon), which makes a single reading unreliable.
  • Some commercial blood tests exist, but results can vary and may not reflect true cellular levels.
  • For now, the most useful approach is to track consistency and how you feel — energy, sleep, clarity — alongside your NAD⁺ routine.


Watch this space: We’re working with pioneering researchers, and reliable NAD⁺ testing may soon become a reality.

How do I know Bio Atelier contains what it claims?

Every Bio Atelier batch undergoes independent third-party testing with published Certificates of Analysis.